The
ILPS is developing a series of commercial methods for lecithin analysis. Until
standardized and adopted by the ILPS & AOCS these methods should
be considered “experimental”. Official
standardized methods of analysis can be found at the ILPS
Standardized Methods for Lecithin Analysis page.
1. A
method is being developed for analysis of phospholipids in lecithin
by HPLC. The method AM101 can be obtained from the Analytical Secretary
Bernd Diehl or the Executive Director Willem van Nieuwenhuyzen. This
method is approved by the DGF German Association of Fat Science as
Official Analytical Method DGF F-I 6a (00). This method determines
the main phospholipids PC, PE, PI, PA, LPC and N-Acyl PE.
2. For
hydrolysed lecithins attempts are made to develop a HPLC method
for quantitative analysis of all the 4lysophospholipids LPC, LPE,
LPI and LPA. Due to baseline fluctuations and insufficient peak
segregation, statistical reproducibility between laboratories failed
so far. Method AM 101 can be used with acceptable repeatability
in laboratories for following e.g. processing.
3. New
HPTLC equipment may encourage the development of a HPTLC method
for the determination of all classes of phospholipids in hydrolyzed
lecithin. Method DGF F-I (99) describes the procedure for standard
lecithin.
4. Standards
can be obtained from Avanti Polar Lipids, USA by contacting www.avantilipids.com or Spectral Service, Germany by contacting info@spectralservice.de. |
